Behaviour of mouse primordial germ cells in the chick embryo.
نویسندگان
چکیده
Hind guts of 9i-day mouse embryos were transplanted into the posterior part of the coelomic cavity of 2|-day chick embryos. The hosts were sacrificed after 1-7 days and the mouse primordial germ cells (PGCs) in the graft and in the surrounding host tissues were searched for by means of the histochemical technique for alkaline phosphatase. Altogether 94 grafts were examined. During the first 3 days of intracoelomic development of the graft accumulations of mouse PGCs close to the mesonephros, the mesentery or the gonad of a chick embryo were observed in 26 out of 51 cases. In 12 grafts single PGCs crossed the boundary between the host and the graft and settled in host tissues such as the mesonephros, the mesentery or the gonad. After 3 days mouse PGCs are no longer visible in the chick tissues. However, the number of PGCs in the grafts also gradually decreases and from the 4th day onwards many of the grafts contain no PGCs. The ability of mouse PGCs to survive extragonadally, even in the embryonic hind gut, is thus limited. In someof the 4-to 7-day-old grafts PGCs occur on the periphery of the graft in the form of single aggregations. From the 6th day the only PGCs which survive are those in aggregations. The experiments indicate that the gonads, together with adjacent tissues (mesonephros, mesentery) of a chick embryo are attractive to mouse primordial germ cells and that the hypothetical attractive substance is not species specific.
منابع مشابه
The effect of BMP4 on mouse embryonic stem cell proliferation and differentiation into primordial germ cells
Background and Aim: Artificial gamete production from stem cells is a novel strategy for treatment of infertility. Among various stem cell sources, embryonic stem cells (ESC) can be considered as an appropriate source for in vitro formation of germ cells. In this study we evaluated the effect of BMP4 on proliferation and differentiation of mouse embryonic stem cells into primordial germ cells (...
متن کاملFunctional Concentrations of BMP4 on Differentiation of Mouse Embryonic Stem Cells to Primordial Germ Cells
متن کامل
Prospects for transgenesis in the chick
Research to develop a useful method for genetic modification of the chick has been on-going since the first demonstrations in the mouse in the 1980s that genetic modification is an invaluable tool for the study of gene function. Manipulation of the chick zygote is possible but inefficient. Considerable progress has been made in developing potentially pluripotent embryo stem cells and their cont...
متن کاملAnalysis of germ line development in the chick embryo using an anti-mouse EC cell antibody.
We have found that EMA-1, a monoclonal antibody originally raised against mouse embryonal carcinoma (Nulli SCC1) cells (Hahnel & Eddy, 1982), also labels chick primordial germ cells (PGCs). We have used this antibody in immunohistological studies to follow the development of PGCs in the chick embryo from the time of their initial appearance beneath the epiblast, through their migratory phase an...
متن کاملAn investigation of primordial germ cell development in embryo and larvae of Huso huso
In sturgeons, Primordial Germ Cells originate from the vegetal pole of the egg. They migrate toward genital ridges during developmental stages and finally differentiate into gametes (the only cells that transmit genetic information). In this study, the cells were isolated from the place of migration of primordial germ cells in Huso huso’s larvae during two stages before and after absorption of ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of embryology and experimental morphology
دوره 25 2 شماره
صفحات -
تاریخ انتشار 1971